Cannot access website-HTTP 404

I tried hosting an access database with a data access page in my previously
functional website, received the following error "Internet Server Error:
Object/Module Not Found", so I messed with some Execute Permissions under the
Home Directory tab in IIS and now my website does not work along with the web
access to the database. Any suggestions?

I went back to put the settings back, but they appeared as if I had never
changed them???
--
Steve
Server 2003 Beginner
.