lost subfolders and email -vista mail

Hi there, I have posted a similar email in browsers and email forum (
thougth it was this one). Yesterday I emptied the junk mail and when I
looked again all my subfolders and email had disappeared. All my
business work. I am lost! I have microsoft security essentials for
antivirus. i have tried looking in the appdata local microsoft
windowsmail folder and they are not there. what can I do now? will they
be anywhere on my hard drive that I can access?