Tracking a memory leak.

I have an object tree that is pretty gigantic and it holds about 100mb of data. When I set the top object to null, I expect that the .NET framework will clean up the memory at some point. However, I am looking at the Task Manager and I don't see the MemUsage column decreasing even after an hour or two. I know that TaskManager may not be the best place to see what is the true way to gauge memory usage and/or presense of memory leaks. So I am looking for a pointer on what I should use to see whether setting an object to null really will at some point free up memory.

Regards
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